Amplitude and frequency of wetting and drying cycles drive N2 and N2O emissions from a subtropical pasture

Abstract

AbstractThis study investigated the effects of irrigation frequency on N2 and N2O emissions from an intensively managed pasture in the subtropics. Irrigation volumes were estimated to replace evapotranspiration and were applied either once (low frequency) or split into four applications (high frequency). To test for legacy effects, a large rainfall event was simulated at the end of the experiment. Over 15 days, 7.9 ± 2.7 kg N2 + N2O-N ha−1 was emitted on average regardless of irrigation frequency, with N2O accounting for 25% of overall N2 + N2O. Repeated, small amounts of irrigation produced an equal amount of N2 + N2O losses as a single, large irrigation event. The increase in N2O emissions after the large rainfall event was smaller in the high-frequency treatment, shifting the N2O/(N2O + N2) ratio towards N2, indicating a treatment legacy effect. Cumulative losses of N2O and N2 did not differ between treatments, but higher CO2 emissions were observed in the high-frequency treatment. Our results suggest that the increase in microbial activity and related O2 consumption in response to small and repeated wetting events can offset the effects of increased soil gas diffusivity on denitrification, explaining the lack of treatment effect on cumulative N2O and N2 emissions and the abundance of N cycling marker genes. The observed legacy effect may be linked to increased mineralisation and subsequent increased dissolved organic carbon availability, suggesting that increased irrigation frequency can reduce the environmental impact (N2O), but not overall magnitude of N2O and N2 emissions from intensively managed pastures.