Investigation of Some Biological Activities of Ajuga chamaepitys subsp. chia

Abstract

Ajuga L., which is an Eastern Mediterranean element, is popularly known as ground pine, dwarf grass, ground cypress, bitter gourd, wormwood, yeast grass (mayasılotu in Turkish) (Zeybek and Zeybek, 1994). Ajuga chamaepitys L. Schreb species has been used since the Middle Ages as a diuretic, antidote for poisonous animal stings, and as an invigorating, wound-healing and diaphoretic plant. This study evaluated antibiofilm, time-kill potential and antioxidant activity of four extracts of A. chamaepitys subsp. chia (Schreb.) Arcang. Crystal violet binding assay were used to determinate antibiofilm activities of extracts against test microorganisms (4 Gram negative, 3 Gram positive and 1yeast). Antioxidant activity was evaluated by using 2,2-diphenyl1-picrylhydrazyl and reducing antioxidant capacity methods. The highest antibiofilm activity was obtained in acetone extract against P. aeruginosa ATCC 27853 and S. aureus ATCC 6538P bacteria. In the time-dependent killing test of plant extracts, microbicidal effect was obtained after 24 hours. The highest antioxidant activity was obtained from plants ethyl acetate extract.