Use of sunflower immature embryos culture in in vitro for fast creation of fertility restorer resistant to tribenuron methyl herbicide

Abstract

Aim. Acceleration of the sunflower lines homozygosity for isolation of the fertility restorer resistant to tribenuron methyl herbicide using in vitro culture of sunflower immature embryos. Methods. Methods of immature sunflower embryos cultivation in in vitro culture; methods of classical crossing. Results. As a result, homozygous genotype was created and isolated from three combinations (BH0118xSURES-2, BH0218xSURES-2 and BH0318xSURES-2) for 10 parent sunflower lines. Conclusions. The use of the technology of sunflower immature embryos cultivation in in vitro culture effectively accelerates the sunflower lines homozygosity for the fertility restorer isolation resistant to tribenuron methyl herbicide. The involvement of immature sunflower embryos cultivation in in vitro culture methods can reduce time for creation of initial parental sunflower lines twice. Keywords: sunflower, immature embryos, in vitro culture, herbicide resistance, tribenuron methyl.