Alleles at the Gli-A3 locus in groups of ukrainian winter common wheat cultivars of different origin

Abstract

Aim. The aim of the study was to analyze the diversity and frequencies of alleles at the minor gliadin locus Gli-A3 in groups of common winter wheat cultivars developed in different breeding institutions of Ukraine in different periods of time. Methods. Acid polyacrylamide gel electrophoresis of gliadins was carried out. To identify the Gli-A3 allele of the cultivar Myronivska storichna, segregation in a sample of F2 seeds from crossing with the cultivar Bezosta 1 was analyzed. Results. Alleles at Gli-A3 were identified in 511 Ukrainian cultivars. In addition to the alleles a-d, a new allele Gli-A3e encoding two omega-gliadins was identified in Myronivska storichna. The alleles a and b predominate in the total group of cultivars. Differences in the allele composition and frequencies were revealed between some groups of cultivars. A significant reduction in the frequency of Gli-A3b was detected for the groups of the Plant Breeding and Genetics Institute (PBGI) and the V. Ya. Yuryev Plant Production Institute (IPP) cultivars released after 2010. Conclusions. The frequencies of the predominant alleles a and b are similar in the groups of the Central Forest-Steppe cultivars in different periods of breeding. An increase in the frequency of the allele a was noted for the groups of PBGI (the Steppe) and IPP (the Eastern Forest-Steppe) cultivars, which may indicate its adaptive value under conditions of an increase in the average annual temperature in these zones.