Mitochondrial Contribution to the Anoxic Ca2+ Signal in Maize Suspension-Cultured Cells

Author:

Subbaiah Chalivendra C.1,Bush Douglas S.2,Sachs Martin M.13

Affiliation:

1. Department of Crop Sciences, University of Illinois, Urbana, Illinois 61801 (C.C.S., M.M.S.)

2. Department of Biological Sciences, Rutgers University, Newark, New Jersey 07102 (D.S.B.)

3. United States Department of Agriculture/Agricultural Research Service, Plant Physiology and Genetics Research Unit, Urbana, Illinois 61801 (M.M.S.)

Abstract

Abstract Anoxia induces a rapid elevation of the cytosolic Ca2+concentration ([Ca2+]cyt) in maize (Zea mays L.) cells, which is caused by the release of the ion from intracellular stores. This anoxic Ca2+ release is important for gene activation and survival in O2-deprived maize seedlings and cells. In this study we examined the contribution of mitochondrial Ca2+ to the anoxic [Ca2+]cyt elevation in maize cells. Imaging of intramitochondrial Ca2+ levels showed that a majority of mitochondria released their Ca2+ in response to anoxia and took up Ca2+ upon reoxygenation. We also investigated whether the mitochondrial Ca2+ release contributed to the increase in [Ca2+]cyt under anoxia. Analysis of the spatial association between anoxic [Ca2+]cytchanges and the distribution of mitochondrial and other intracellular Ca2+ stores revealed that the largest [Ca2+]cyt increases occurred close to mitochondria and away from the tonoplast. In addition, carbonylcyanide p-trifluoromethoxyphenyl hydrazone treatment depolarized mitochondria and caused a mild elevation of [Ca2+]cyt under aerobic conditions but prevented a [Ca2+]cyt increase in response to a subsequent anoxic pulse. These results suggest that mitochondria play an important role in the anoxic elevation of [Ca2+]cyt and participate in the signaling of O2 deprivation.

Publisher

Oxford University Press (OUP)

Subject

Plant Science,Genetics,Physiology

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