Enhanced Low Oxygen Survival in Arabidopsis through Increased Metabolic Flux in the Fermentative Pathway-Reference-Cited by-同舟云学术

Enhanced Low Oxygen Survival in Arabidopsis through Increased Metabolic Flux in the Fermentative Pathway

Published:2003-07-01 Issue:3 Volume:132 Page:1292-1302
ISSN:1532-2548
Container-title:Plant Physiology
language:en
Short-container-title:

Author:

Ismond Kathleen P.¹,Dolferus Rudy¹,De Pauw Mary¹,Dennis Elizabeth S.¹,Good Allen G.¹

Affiliation:

1. Department of Biological Sciences, University of Alberta, Edmonton, Alberta, Canada T6G 2E9 (K.P.I., M.D.P., A.G.G.); and Division of Plant Industry, Commonwealth Scientific and Industrial Research Organization, G.P.O. Box 1600, Canberra, Australian Capital Territory 2601, Australia (R.D., E.S.D.)

Abstract

Abstract We manipulated the enzyme activity levels of the alcohol fermentation pathway, pyruvate decarboxylase (PDC), and alcohol dehydrogenase (ADH) in Arabidopsis using sense and antisense overexpression of the corresponding genes (PDC1, PDC2, and ADH1). Transgenic plants were analyzed for levels of fermentation and evaluated for changes in hypoxic survival. Overexpression of either Arabidopsis PDC1 or PDC2 resulted in improved plant survival. In contrast, overexpression of Arabidopsis ADH1 had no effect on flooding survival. These results support the role of PDC as the control step in ethanol fermentation. Although ADH1 null mutants had decreased hypoxic survival, attempts to reduce the level of PDC activity enough to see an effect on plant survival met with limited success. The combination of flooding survival data and metabolite analysis allows identification of critical metabolic flux points. This information can be used to design transgenic strategies to improve hypoxic tolerance in plants.

Publisher

Oxford University Press (OUP)

Subject

Plant Science,Genetics,Physiology

Link

http://academic.oup.com/plphys/article-pdf/132/3/1292/38699498/plphys_v132_3_1292.pdf

Reference66 articles.

1. Bouny JM, Saglio PH (1996) Glycolytic flux and hexokinase activities in anoxic maize root tips acclimated by hypoxic pre-treatment. Plant Physiol 111 : 187–194

2. Boyer JS (1982) Plant productivity and environment. Science 218 : 443–448

3. Braun KP, Cody RB, Jones JDR, Peterson CM (1995) A structural assignment for a stable acetaldehyde-lysine adduct. J Biol Chem 270 : 11263–11266

4. Bucher M, Brändle R, Kuhlemeier C (1994) Ethanolic fermentation in transgenic tobacco expressing Zymomonas mobilis pyruvate decarboxylase. EMBO J 13 : 2755–2763

5. Davies DD (1980) Anaerobic metabolism and production of organic acids. In PK Stumpf, EE Conn, eds, The Biochemistry of Plants: A Comprehensive Treatise, Vol 2. Academic Press, New York, pp 581–611

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