Identification of Arabidopsis rat Mutants

Author:

Zhu Yanmin1,Nam Jaesung1,Humara Jaime M.1,Mysore Kirankumar S.1,Lee Lan-Ying1,Cao Hongbin1,Valentine Lisa1,Li Jingling1,Kaiser Anthony D.1,Kopecky Andrea L.1,Hwang Hau-Hsuan1,Bhattacharjee Saikat1,Rao Praveen K.1,Tzfira Tzvi1,Rajagopal Jyothi1,Yi HoChul1,Veena 1,Yadav Badam S.1,Crane Yan M.1,Lin Kui1,Larcher Yves1,Gelvin Matthew J.K.1,Knue Marnie1,Ramos Cynthia1,Zhao Xiaowen1,Davis Susan J.1,Kim Sang-Ic1,Ranjith-Kumar C.T.1,Choi Yoo-Jin1,Hallan Vipin K.1,Chattopadhyay Sudip1,Sui Xiangzhen1,Ziemienowicz Alicja1,Matthysse Ann G.1,Citovsky Vitaly1,Hohn Barbara1,Gelvin Stanton B.1

Affiliation:

1. Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907–1392 (Y.Z., J.N., J.M.H., K.S.M., L.-Y.L., H.C., J.L., Ant. D.K., And. L.K., H.-H.H., S.B., P.K.R., J.R., H.Y., V., B.S.Y., Y.M.C., K.L., Y.L., M.J.K.G., M.K., X.Z., S.J.D., S.-I.K., C.T.R.-K., V.K.H., S.C., X.S., S.B.G.); Faculty of Natural Resources and Life Sciences, Dong-A University, Pusan 604–714, South Kore

Abstract

Abstract Limited knowledge currently exists regarding the roles of plant genes and proteins in the Agrobacterium tumefaciens-mediated transformation process. To understand the host contribution to transformation, we carried out root-based transformation assays to identify Arabidopsis mutants that are resistant to Agrobacterium transformation (rat mutants). To date, we have identified 126 rat mutants by screening libraries of T-DNA insertion mutants and by using various “reverse genetic” approaches. These mutants disrupt expression of genes of numerous categories, including chromatin structural and remodeling genes, and genes encoding proteins implicated in nuclear targeting, cell wall structure and metabolism, cytoskeleton structure and function, and signal transduction. Here, we present an update on the identification and characterization of these rat mutants.

Publisher

Oxford University Press (OUP)

Subject

Plant Science,Genetics,Physiology

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