The Expression of D-Cyclin Genes Defines Distinct Developmental Zones in Snapdragon Apical Meristems and Is Locally Regulated by the Cycloidea Gene

Abstract

Abstract Three D-cyclin genes are expressed in the apical meristems of snapdragon (Antirrhinum majus). The cyclin D1 andD3b genes are expressed throughout meristems, whereascyclin D3a is restricted to the peripheral region of the meristem, especially the organ primordia. During floral development,cyclin D3b expression is: (a) locally modulated in the cells immediately surrounding the base of organ primordia, defining a zone between lateral organs that may act as a developmental boundary; (b) locally modulated in the ventral petals during petal folding; and (c) is specifically repressed in the dorsal stamen by thecycloidea gene. Expression of both cyclin D3 genes is reduced prior to the cessation of cell cycle activity, as judged by histone H4 expression. Expression of all three D-cyclin genes is modulated by factors that regulate plant growth, particularly sucrose and cytokinin. These observations may provide a molecular basis for understanding the local regulation of cell proliferation during plant growth and development.