Global Characterization of Cell-Specific Gene Expression through Fluorescence-Activated Sorting of Nuclei-Reference-Cited by-同舟云学术

Global Characterization of Cell-Specific Gene Expression through Fluorescence-Activated Sorting of Nuclei

Published:2008-03-19 Issue:1 Volume:147 Page:30-40
ISSN:1532-2548
Container-title:Plant Physiology
language:en
Short-container-title:

Author:

Zhang Changqing¹,Barthelson Roger A.¹,Lambert Georgina M.¹,Galbraith David W.¹

Affiliation:

1. Department of Plant Sciences (C.Z., R.A.B., G.M.L., D.W.G.), and BIO5 Institute for Collaborative Bioresearch (R.A.B., G.M.L., D.W.G.), The University of Arizona, Tucson, Arizona 85721

Abstract

AbstractWe describe a simple and highly effective means for global identification of genes that are expressed within specific cell types within complex tissues. It involves transgenic expression of nuclear-targeted green fluorescent protein in a cell-type-specific manner. The fluorescent nuclei are then purified from homogenates by fluorescence-activated sorting, and the RNAs employed as targets for microarray hybridization. We demonstrate the validity of the approach through the identification of 12 genes that are selectively expressed in phloem.

Publisher

Oxford University Press (OUP)

Subject

Plant Science,Genetics,Physiology

Link

http://academic.oup.com/plphys/article-pdf/147/1/30/38101925/plphys_v147_1_30.pdf

Reference41 articles.

1. Comparison of the contributions of the nuclear and cytoplasmic compartments to global gene expression in human cells

2. Apical organization and maturation of the cortex and vascular cylinder inArabidopsis thaliana (Brassicaceae) roots

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4. A Gene Expression Map of the Arabidopsis Root

5. A High-Resolution Root Spatiotemporal Map Reveals Dominant Expression Patterns

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