Global Characterization of Cell-Specific Gene Expression through Fluorescence-Activated Sorting of Nuclei-Reference-Cited by-同舟云学术

Global Characterization of Cell-Specific Gene Expression through Fluorescence-Activated Sorting of Nuclei

Published:2008-03-19 Issue:1 Volume:147 Page:30-40
ISSN:1532-2548
Container-title:Plant Physiology
language:en
Short-container-title:

Author:

Zhang Changqing¹,Barthelson Roger A.¹,Lambert Georgina M.¹,Galbraith David W.¹

Affiliation:

1. Department of Plant Sciences (C.Z., R.A.B., G.M.L., D.W.G.), and BIO5 Institute for Collaborative Bioresearch (R.A.B., G.M.L., D.W.G.), The University of Arizona, Tucson, Arizona 85721

Abstract

AbstractWe describe a simple and highly effective means for global identification of genes that are expressed within specific cell types within complex tissues. It involves transgenic expression of nuclear-targeted green fluorescent protein in a cell-type-specific manner. The fluorescent nuclei are then purified from homogenates by fluorescence-activated sorting, and the RNAs employed as targets for microarray hybridization. We demonstrate the validity of the approach through the identification of 12 genes that are selectively expressed in phloem.

Publisher

Oxford University Press (OUP)

Subject

Plant Science,Genetics,Physiology

Link

http://academic.oup.com/plphys/article-pdf/147/1/30/38101925/plphys_v147_1_30.pdf

Reference41 articles.

1. Comparison of the contributions of the nuclear and cytoplasmic compartments to global gene expression in human cells

2. Apical organization and maturation of the cortex and vascular cylinder inArabidopsis thaliana (Brassicaceae) roots

3. We've got mail!

4. A Gene Expression Map of the Arabidopsis Root

5. A High-Resolution Root Spatiotemporal Map Reveals Dominant Expression Patterns

Cited by 110 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. The stem cell-type transcriptome of bioenergy sorghum reveals the spatial regulation of secondary cell wall networks;2023-04-25

2. Companion Cell and Sieve Elements;Encyclopedia of Life Sciences;2023-03-30

3. Loss of linker histone H1 in the maternal genome influences DEMETER-mediated demethylation and affects the endosperm DNA methylation landscape;Frontiers in Plant Science;2022-12-22

4. A root phloem pole cell atlas reveals common transcriptional states in protophloem-adjacent cells;Nature Plants;2022-08-04

5. Deciphering Precise Gene Transcriptional Expression Using gwINTACT in Tomato;Frontiers in Plant Science;2022-04-14