The Arabidopsis MutantjasonProduces Unreduced First Division Restitution Male Gametes through a Parallel/Fused Spindle Mechanism in Meiosis II

Abstract

AbstractIn plants, whole-genome doubling (polyploidization) is a widely occurring process largely contributing to plant evolution and diversification. The generation and fusion of diploid gametes is now considered the major route of plant polyploidization. The parallel arrangement or fusion of meiosis II MII spindles (ps) is one of the most frequently reported mechanisms generating triploid offspring. Through a forward genetics screen of an Arabidopsis (Arabidopsis thaliana) ethyl methanesulfonate population, we identified Arabidopsis thaliana Parallel Spindles1 (AtPS1), which was recently reported as a major gene implicated in the control of the ps meiotic defect. In addition, we describe the isolation and characterization of a novel allele of JASON, involved in male gametophytic ploidy regulation in plants. Similar to atps1 mutants, jason produces more than 25% 2n pollen grains and spontaneously forms triploid offspring. By combining both cytological and genetic approaches, we demonstrate that loss of JASON causes the formation of parallel arranged and fused spindles in male MII, resulting in the production of unreduced first division restitution 2n spores. Although JASON encodes a protein of unknown function, we additionally show that the meiotic ps defect in jason is caused by a reduction in AtPS1 transcript levels, indicating that JASON positively regulates AtPS1 expression, allowing the proper organization and orientation of metaphase II spindle plates in MII.