The Intracellular Fate of a Recombinant Protein Is Tissue Dependent

Author:

Drakakaki Georgia1,Marcel Sylvain1,Arcalis Elsa1,Altmann Friedrich1,Gonzalez-Melendi Pablo1,Fischer Rainer1,Christou Paul1,Stoger Eva1

Affiliation:

1. Institute for Molecular Biotechnology, Biology VII, Aachen University, 52074 Aachen, Germany (G.D., S.M., E.A., R.F., E.S.); Department for Chemistry, Glycobiology Division, University of Natural Resources and Applied Life Sciences, 1190 Vienna, Austria (F.A.); Centro de Investigaciones Biologicas, Consejo Superior de Investigaciones Científicas, 28040 Madrid, Spain (P.G.-M.); and Department de

Abstract

Abstract Recombinant proteins directed to the secretory pathway in plants require a signal peptide for entry into the endoplasmic reticulum. In the absence of further targeting information, such proteins are generally secreted via the default pathway to the apoplast. This has been well documented in protoplasts and leaf tissue, but the trafficking of recombinant proteins in seeds and other storage tissues has rarely been investigated. We used Aspergillus niger phytase as a model glycoprotein to compare the intracellular fate of a recombinant protein in the leaves and seeds of rice (Oryza sativa). Using fluorescence and electron microscopy we showed that the recombinant protein was efficiently secreted from leaf cells as expected. In contrast, within endosperm cells it was retained in endoplasmic reticulum-derived prolamin bodies and protein storage vacuoles. Consistent with our immunolocalization data, the phytase produced in endosperm cells possessed oligomannose and vacuolar-type N-glycans [Man3(Xyl)(Fuc)GlcNAc2], whereas the phytase produced in leaves contained predominantly secretion-type N-glycans [GlcNAc2Man3(Xyl)(Fuc)GlcNAc2]. The latter could not be detected in preparations of the endosperm-derived phytase. Our results show that the intracellular deposition and modification of a recombinant protein is tissue dependent.

Publisher

Oxford University Press (OUP)

Subject

Plant Science,Genetics,Physiology

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