Affiliation:
1. Center for Plant Environmental Stress Physiology, 1165 Horticulture Building, Purdue University, West Lafayette, Indiana 47907–1165 (Z.G., H.K., T.K.M., J.Z., R.A.B., P.M.H.);
2. Department of Biochemistry MS200, University of Nevada, Reno, Nevada 89557–0014 (M.A.C., S.K., J.C.C.); and
3. Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, Oklahoma 74078 (A.R., D.B.)
Abstract
Abstract
Repetitive rounds of differential subtraction screening, followed by nucleotide sequence determination and northern-blot analysis, identified 84 salt-regulated (160 mm NaCl for 4 h) genes in Arabidopsis wild-type (Col-0 gl1) seedlings. Probes corresponding to these 84 genes and ACP1,RD22BP1, MYB2, STZ, andPAL were included in an analysis of salt responsive gene expression profiles in gl1 and the salt-hypersensitive mutant sos3. Six of 89 genes were expressed differentially in wild-type and sos3 seedlings; steady-state mRNA abundance of five genes (AD06C08/unknown, AD05E05/vegetative storage protein 2 [VSP2],AD05B11/S-adenosyl-l-Met:salicylic acid carboxyl methyltransferase [SAMT],AD03D05/cold regulated 6.6/inducible2 [COR6.6/KIN2], and salt tolerance zinc finger [STZ]) was induced and the abundance of one gene (AD05C10/circadian rhythm-RNA binding1 [CCR1]) was reduced in wild-type plants after salt treatment. The expression of CCR1, SAMT,COR6.6/KIN2, and STZ was higher insos3 than in wild type, and VSP2 andAD06C08/unknown was lower in the mutant. Salt-induced expression of VSP2 in sos1 was similar to wild type, and AD06C08/unknown, CCR1,SAMT, COR6.6/KIN2, and STZwere similar to sos3. VSP2 is regulated presumably bySOS2/3 independent of SOS1, whereas the expression of the others is SOS1 dependent.AD06C08/unknown and VSP2 are postulated to be effectors of salt tolerance whereas CCR1,SAMT, COR6.6/KIN2, and STZare determinants that must be negatively regulated during salt adaptation. The pivotal function of the SOS signal pathway to mediate ion homeostasis and salt tolerance implicatesAD06C08/unknown, VSP2,SAMT, 6.6/KIN2, STZ, andCCR1 as determinates that are involved in salt adaptation.
Publisher
Oxford University Press (OUP)
Subject
Plant Science,Genetics,Physiology
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