Biocompatible Fluids for Use in Micro Total Analysis Systems

Abstract

The trend in micro total analysis systems is favouring the segmented flow approach as it has the potential to reduce contamination issues and allow for a greater quantity of samples to be processed in any given time frame. With this trend comes the need for biocompatible fluids to separate the bio-samples. To date a number of authors have cited different fluids for this purpose, some noting good results and some noting total inhibition due to the segmenting fluid. This paper aims to demonstrate the extent of reduced efficiency for a number of potential segmenting fluids. A number of PCR samples are thermally cycled in a commercial PCR machine after having been in contact with a variety of potential segmenting fluids. The resulting efficiencies and crossing thresholds are then compared to determine the effects on the bio-samples. This paper details the first quantitative test of PCR compatibility with segmenting fluids. Such work is critical to the development of this technology in an efficient manner. The experimental results of this work are promising for the future of the segmented flow approach to micro-Total Analysis Systems.