Effect of Hydroxyapatite Nanoparticles on Biotransport Phenomena in Freezing HeLa Cells

Author:

Yi Jingru1,Zhao Gang12

Affiliation:

1. Department of Electronic Science and Technology, University of Science and Technology of China, Road JinZhai 96, Hefei 230027, China

2. Anhui Provincial Engineering Technology Research Center for Biopreservation and Artificial Organs, Hefei 230027, China e-mail:

Abstract

The effect of nanoparticles on subzero biotransport phenomena of living cells is very rare in the literature, although the information is of great importance for the application of nanotechnology in the field of cryobiology. In this study, subzero water transport phenomena in freezing HeLa cells in 1 × phosphate buffered saline (PBS) containing 0%, 0.05%, and 0.1% (w/w) hydroxyapatite (HA) nanoparticles with and without pre-incubation at 37 °C was quantitatively investigated. The results reveal that the presence of HA nanoparticles slightly facilitates the subzero water transport of HeLa cells.

Publisher

ASME International

Subject

Electrical and Electronic Engineering,General Materials Science,General Medicine

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