Graphene Nanoparticle Modified Laser Engraved Kapton Sensor for Environmental Estrogen Detection

Abstract

Abstract In this study, we developed a graphene-based flexible electrochemical immune sensor to rapidly detect 17β-estradiol in phosphate buffer saline (PBS) buffer solution. The irreversible oxidation of the molecule’s phenol group served as the basis for the electrochemical determination of 17β-estradiol. The calculated incubation time of the laser-engraved Kapton sensor with modified graphene polyaniline ink (LEK/G-PANI) is 30 minutes, and the optimum PH level is 7.4. The proposed immune sensor has impedance change with excellent linearity from 0.61 pg/ml to 156.3 pg/ml with a limit of detection (LOD) is 3.54 pg/ml(S/N = 3) when testing17β-estradiol in PBS (1x) buffer solution (pH = 7.4).