Low Intensity Ultrasound Induces Epithelial Cell Adhesion Responses-Reference-Cited by-同舟云学术

Low Intensity Ultrasound Induces Epithelial Cell Adhesion Responses

Published:2020-05-14 Issue:9 Volume:142 Page:
ISSN:0148-0731
Container-title:Journal of Biomechanical Engineering
language:en
Short-container-title:

Author:

Lim Jormay¹,Chu Yeh-Shiu²,Chu Ya-Cherng¹,Lo Chun-Min³,Wang Jaw-Lin⁴

Affiliation:

1. Department of Biomedical Engineering, National Taiwan University, 605 Jen-Su Hall, 1 Section 4, Roosevelt Road, Taipei 10617, Taiwan

2. Brian Research Center, National Yang-Ming University, No. 155, Sec. 2, Linong Street, Taipei 112, Taiwan

3. Department of Biomedical Engineering, National Yang-Ming University, No. 155, Sec. 2, Linong Street, Taipei 112, Taiwan

4. Department of Biomedical Engineering, National Taiwan University, 602 Jen-Su Hall, 1 Section 4, Roosevelt Road, Taipei 10617, Taiwan

Abstract

Abstract In this study, we investigated the cellular mechanosensitive responses to a low intensity ultrasound (LIUS) stimulation (ISATA = 1 mW/cm2, pressure = 10 kPa). The dose and temporal effects at cell–substrate adhesion (CSA) at the basal level and cell–cell adhesion (CCA) at the apical level are reported in detail. A model of mouse mammary gland epithelial cells (EpH4) and the phosphorylation of mechanosensitive 130 kDa Crk-associated substrate (p130CAS) as an indicator for cellular responses were used. The intensity of phospho-p130CAS was found to be dependent on LIUS stress level, and the p130CAS was phosphorylated after 1 min stimulation at CSA. The phospho-p130CAS was also found to increase significantly at CCA upon LIUS stimulation. We confirmed that the cellular responses to ultrasound are immediate and dose dependent. Ultrasound affects not only CSA but also CCA. An E-cadherin knockout (EpH4ECad−/−) model also confirmed that phosphorylation of p130CAS at CCA is related to E-cadherins.

Funder

Ministry of Science and Technology

National Health Research Institute

National Taiwan University

Publisher

ASME International

Subject

Physiology (medical),Biomedical Engineering

Link

http://asmedigitalcollection.asme.org/biomechanical/article-pdf/doi/10.1115/1.4046883/6534848/bio_142_09_091014.pdf

Reference40 articles.

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