Microfluidic System for Rapid Enumeration and Detection of Microparticles

Author:

Sen A. K.1,Bhardwaj P.2

Affiliation:

1. Department of Mechanical Engineering, Indian Institute of Technology Madras, Chennai 600 036, India

2. Department of Mechanical Engineering, Indian Institute of Technology Guwahati, Guwahati 781039, India

Abstract

A microfluidic system for rapid concentration, enumeration, and size based detection of microparticles is presented. The system includes a micro flow cytometer chip together with fluidics, optics and control on a single platform. The micro flow cytometer chip was designed, fabricated, and integrated with fluidics and optical fibers. The flow microchannel employs chevron structures at the top and bottom surfaces of the channel to achieve two-dimensional flow focusing. The system employs a cross-flow filter for sample concentration thus enabling enumeration and detection of microparticles even at low concentration levels (∼1.1 × 104/ml). A flow stabilizer chip based on the concept of a fluid chamber with a flexible membrane as the top wall was used to reduce flow pulsations within the fluidic system thus improving measurement accuracy. The excitation optical fiber is connected to a laser source and the collection fibers are connected to photomultiplier tubes (PMTs) for signal manipulation and conversion. Labview was used for data acquisition through a PC interface. The ability of the system for enumeration and size-based detection of microparticles was demonstrated using polystyrene microbeads suspended in PBS as the sample.

Publisher

ASME International

Subject

Mechanical Engineering

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3