Blood Freezing to Nearly Absolute Zero Temperature: −272.29°C-Reference-Cited by-同舟云学术

Blood Freezing to Nearly Absolute Zero Temperature: −272.29°C

Published:1981-02-01 Issue:1 Volume:103 Page:24-26
ISSN:0148-0731
Container-title:Journal of Biomechanical Engineering
language:en
Short-container-title:

Author:

Cravalho E. G.¹,Huggins C. E.²,Diller K. R.³,Watson W. W.⁴

Affiliation:

1. Harvard/MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, Mass. 02139

2. Department of Surgery, Harvard Medical School, Boston, Mass. 02139, and Blood Bank and Transfusion Service, Massachusetts General Hospital, Boston, Mass. 02114

3. Bio-Heat Transfer Laboratory, Biomedical Engineering Center, The University of Texas at Austin, Austin, Tex. 78712

4. Cryogenic Engineering Laboratory, Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, Mass. 02139

Abstract

Human erythrocytes were collected in ACD anticoagulant, glycerolized with an equal volume of 8.6 M glycerol, 8 percent glucose, 1 percent fructose, and 0.3 percent Na2 EDTA solution, sealed in glass ampules, and frozen to −272.29°C (0.86°K) in liquid helium under high vacuum. Biological assays performed after thawing and three-stage dialysis to remove the glycerol showed less than 1 percent hemolysis. Intracellular potassium, 2,3 DPG, and ATP were within the normal range.

Publisher

ASME International

Subject

Physiology (medical),Biomedical Engineering

Link

http://asmedigitalcollection.asme.org/biomechanical/article-pdf/103/1/24/5647963/24_1.pdf

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1. Preservation of Biological Material by Freezing and Thawing;Heat Transfer in Medicine and Biology;1985