An in Vitro Model of Neural Trauma: Device Characterization and Calcium Response to Mechanical Stretch
Author:
Geddes Donna M.1, Cargill Robert S.2
Affiliation:
1. School of Biomedical Engineering, Georgia Institute of Technology, Atlanta, GA 30332-0405 2. School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, GA 30332-0405
Abstract
An in vitro model for neural trauma was characterized and validated. The model is based on a novel device that is capable of applying high strain rate, homogeneous, and equibiaxial deformation to neural cells in culture. The deformation waveform is fully arbitrary and controlled via closed-loop feedback. Intracellular calcium [Ca2+]i alterations were recorded in real time throughout the imposed strain with an epifluorescent microscopy system. Peak change in [Ca2+]i, recovery of [Ca2+]i, and percent responding NG108-15 cells were shown to be dependent on strain rate (1−1 to 10−1) and magnitude (0.1 to 0.3 Green’s Strain). These measures were also shown to depend significantly on the interaction between strain rate and magnitude. This model for neural trauma is a robust system that can be used to investigate the cellular tolerance and response to traumatic brain injury.
Publisher
ASME International
Subject
Physiology (medical),Biomedical Engineering
Reference28 articles.
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