Biological Activity of rhBMP-2 Released From PLGA Microspheres-Reference-Cited by-同舟云学术

Biological Activity of rhBMP-2 Released From PLGA Microspheres

Published:2000-02-06 Issue:3 Volume:122 Page:289-292
ISSN:0148-0731
Container-title:Journal of Biomechanical Engineering
language:en
Short-container-title:

Author:

Oldham, J. B.¹,Lu, L.¹,Zhu, and X.¹,Porter B. D.¹,Hefferan T. E.²,Larson D. R.³,Currier B. L.¹,Mikos A. G.⁴,Yaszemski M. J.¹

Affiliation:

1. Departments of Orthopedic Surgery and Bioengineering, Mayo Clinic, 200 First Street SW, Rochester, MN 55905

2. Department of Protein Chemistry and Molecular Biology, Mayo Clinic, 200 First Street SW, Rochester, MN 55905

3. Department of Biostatistics, Mayo Clinic, 200 First Street SW, Rochester, MN 55905

4. Department of Bioengineering, Rice University, Houston, TX 77005

Abstract

Human recombinant bone morphogenetic protein-2 (rhBMP-2) has been proven effective in stimulating the regeneration of bone in both skeletal and extraskeletal locations. Through encapsulation within, and release from, biodegradable poly(DL-lactic-co-glycolic acid) (PLGA) microspheres, a proven vehicle for sustained delivery of various proteins, the local concentrations of rhBMP-2 could be maintained at optimal levels to stimulate bone regeneration and remodeling at the site of healing in diverse clinical settings. Thus the purpose of this work was to investigate the encapsulation of rhBMP-2 in PLGA microspheres and its biologic activity upon release. Using in vitro tests in simulated body fluids, the effect of rhBMP-2 released from PLGA microspheres upon osteoblast cell cultures was found to be statistically similar to the effect produced by positive controls consisting of nonencapsulated aqueous rhBMP-2 in simulated body fluids. This clarifies an important step in skeletal tissue engineering strategies aimed at the use of encapsulated rhBMP-2 to stimulate bone regeneration and remodeling. [S0148-0731(00)01303-0]

Publisher

ASME International

Subject

Physiology (medical),Biomedical Engineering

Link

http://asmedigitalcollection.asme.org/biomechanical/article-pdf/122/3/289/5592278/283_1.pdf

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