Mechanism of lignin degradation via white rot fungi explored using spectral analysis and gas chromatography-mass spectrometry

Abstract

The mechanism of lignin degradation via white rot fungi was studied. Phanerochaete chrysosporium and Pleurotus ostreatus were used for all the experiments, i.e., measuring the concentration and structure of alkali lignins and studying the effect of the substrate concentration and enzyme activity on the removal. Gas chromatography-mass spectrometry was performed on the reaction liquid of the lignin degradation enzyme system. Alkali lignin had a characteristic absorption spectrum with a peak at approximately 280 nm. Precipitation in the laccase (Lac) degradation system occurred earlier, as well as being more obvious than that in the manganese peroxidase (Mnp) degradation system. The maximum removal was 29.4% in the Mnp degradation system at a concentration of 40 mg/L. The removal increased in a concentration-dependent manner in the Lac degradation system. The increase in Mnp and Lac enzyme activity led to an increased alkali lignin removal. The removal of the control group was significantly lower than the experimental degradation systems. The degradation mainly produced organic acids, esters, and aromatic substances. In conclusion, white rot fungi could effectively remove alkali lignin, in which precipitation played a major role, followed by enzymolysis; the enzymolysis was associated with the alkali lignin concentration and enzyme activity.