Resolving species delimitation within the genus Bunopus Blanford, 1874 (Squamata: Gekkonidae) in Iran using DNA barcoding approach

Author:

KHOSRAVANI AZAR,RASTEGAR-POUYANI ESKANDAR,RASTEGAR-POUYANI NASRULLAH,ORAIE HAMZEH,PAPENFUSS THEODORE J.

Abstract

Mitochondrial COI sequences were used to investigate species delimitation within the genus Bunopus in Iran. A dataset with a final sequence length of 633 nucleotides including 100 specimens from 31 geographically distant localities across Iran were generated. The result demonstrated that two major clades with strong support can be identified within the genus Bunopus in Iran. Clade A includes Bunopus crassicaudus and two new entities, eastern populations (subclade A2,1) and Shahdad populations (subclade A2,2). The second clade comprises western and southwestern populations (subclade B1,1), Arabian populations (subclade B1,2) and south and southeast populations in Iran, to which Bunopus tuberculatus (subclade B2) is assigned. In addition to Bunopus crassicaudus and B. tuberculatus, three new candidate species in Iran can easily be identified based on the DNA barcoding approach. 

Publisher

Magnolia Press

Subject

Animal Science and Zoology,Ecology, Evolution, Behavior and Systematics

Reference1 articles.

1.

Anderson, S.C. (1999) The Lizards of Iran. Society for the Study of Amphibians and Reptiles, Ithaca, New York.

Bauer, A.M., Masroor, R., Mcquilla, J.T., Heinick, M.P., Daza, J.D. & Jackman, T.R. (2013) A preliminary phylogeny of the Palearctic naked-toed geckos (Reptilia: Squamata: Gekkonidae) with taxonomic implications. Zootaxa, 3599 (4), 301–324. http://dx.doi.org/10.11646/zootaxa.3599.4.1.

Arnold, E.N. (1980) The scientific results of the Oman flora and fauna survey 1977 (Dhofar). The reptiles and amphibians of Dhofar, Southern Arabia. Journal of Oman Studies Special Report, (No. 2), 273–332.

Blagoev, G.A., Nikolova, N.I., Sobel, C.N., Hebert, P.D.N. & Adamowicz, S.J. (2013) Spiders (Araneae) of Churchill, Manitoba: DNA barcodes and morphology reveal high species diversity and new Canadian records. BMC Ecology, 13, 44. pmid:24279427.

http://dx.doi.org/10.1186/1472-6785-13-44.

Blanford, W.T. (1874) Descriptions of new lizards from Persia and Baluchistàn. Annals and Magazine of Natural History, series 4, 13, 453–455.

https://doi.org/10.1080/00222937408680903

Červenka, J., Kratochvíl, L. & Frynta, D. (2008) Phylogenetic relationships of the gecko genus Carinatogecko (Reptilia: Gekkonidae). Zootaxa, 2636, 59–64.

Červenka, J., Frynta, D. & Kratochvíl, L. (2010) Phylogeny and taxonomy of the Middle Eastern geckos of the genus Cyrtopodion and their selected relatives. Zootaxa, 1931, 25–36.

Darriba, D., Taboada, G.L., Doallo, R. & Posada, D. (2012) j Model Test 2: more models, new heuristics and parallel computing. Nature Methods, 9, 772.

http://dx.doi.org/10.1038/nmeth.2109.

De Queiroz K. (2007) Species concepts and species delimitation. Systematic Biology, 56, 879–886.

https://doi.org/10.1080/10635150701701083

Disi, A.M. & Böhme, W. (1996) Zoogeography of the amphibians and reptiles of Syria, with additional new records. Herpetozoa, 9, 63–70.

Folmer, O., Black, M., Hoeh, W., Lutz R. & Vrijenhoek, R. (1994) DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology, 3, 294–299.

Gamble, T., Greenbaum, E., Russell, A.P., Jackman, T.R. & Bauer, A.M. (2012) Repeated origin and loss of toepads in geckos. Plos One, 7, e39429.

http://dx.doi.org/10.1371/journal. pone.0039429.

Hall, T.A. (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95 ⁄ 98 ⁄ NT. Nucleic Acids Symposium Series, 41, 95–98.

Hausmann, A., Godfray, H.C.J., Huemer P., Mutanen M., Rougerie R., van Nieukerken E.J., Sujeevan Ratnasingham S. & Hebert P.D.N. (2013) Genetic patterns in European geometrid moths revealed by the Barcode Index Number (BIN) system. PLoS ONE, 8, e84518.

http://dx.doi.org/10.1371/journal.pone.0084518

Hebert, P.D.N., Cywinska, A., Ball, S.L. & De Waard, J.R. (2003a) Biological identifications through DNA barcodes. Proceedings of the Royal Society B, 270, 313–321.

https://doi.org/10.1098/rspb.2002.2218

Hebert, P.D.N., Ratnasingham, S. & De Waard, J.R. (2003b) Barcoding animal life: cytochrome c oxidase subunit 1 divergences among closely related species. Proceedings of the Royal Society B, 270 (Suppl.), S96–99.

https://doi.org/10.1098/rsbl.2003.0025

Hebert, P.D.N., Penton, E.H., Burns, J.M., Janzen, D.H. & Hallwachs, W. (2004a) Ten species in one: DNA barcoding reveals cryptic species in the neotropical skipper butterfly Astraptes fulgerator. Proceedings of the National Academy of Science, 101, 14812–14817.

https://doi.org/10.1073/pnas.0406166101

Kapli, P., Botoni, D., Ilgaz, C., Kumlutaş, Y., Avcı, A., Rastegar-Pouyani, N., Fathinia, B., Lymberakis, P., Ahmadzadeh, F. & Poulakakis, N. (2013) Molecular phylogeny and historical biogeography of the Anatolian lizard Apathya (Squamata, Lacertidae). Molecular Phylogenetics and Evolution, 66, 992–1001.

http://dx.doi.org/10.1016/j.ympev.2012.12.002.

Khosravani, A., Rastegar-Pouyani, N., Rastegar-Pouyani, E. & Oraie, H. (2015) Geographic variation of morphological characters in Bunopus tuberculatus Blanford 1874 (Squamata: Gekkonidae) in Iran. Herpetologica, 71, 152–159.

https://doi.org/10.1655/HERPETOLOGICA-D-14-00043.

Leviton, A.E., Anderson, S.C., Adler, K. & Minton, S.A. (1992) Handbook to Middle East Amphibians and Reptiles. Society for the Study of Amphibians and Reptiles, Oxford, Ohio.

Macey, J.R., Wang, Y., Ananjeva, N.B., Larson, A. & Papenfuss, T.J. (1999) Vicariant patterns of fragmentation among Gekkonid lizards of the genus Teratoscincus produced by the Indian collision: a molecular phylogenetic perspective and an area cladogram for central Asia. Molecular Phylogenetics and Evolution, 12, 320–332.

https://doi.org/10.1006/mpev.1999.0641

Macey, J.R., Fong, J.J., Kuehl, J.V., ShaWei S., Ananjeva N.B., Papenfuss, T.J. & Boore, J.J. (2005) The complete mitochondrial genome of a gecko and the phylogenetic position of the Middle Eastern Teratoscincus keyserlingii. Molecular Phylogenetics and Evolution, 36, 188–19.

https://doi.org/10.1016/j.ympev.2005.03.025

Mayden, R.L. (1997) A hierarchy of species concepts: The denouement in the saga of the species problem. In: Claridge, M.F., Dawah, H.A. & Wilson, M.R. (Eds.), Species: The Units of Biodiversity. Chapman and Hall, London. pp. 381–424.

Mayr, E. (1982) The Growth of Biological Thought: Diversity, Evolution, and Inheritance. Belknap Press of Harvard University Press, Cambridge, Massachusetts.

Meyer, C.P & Paulay, G. (2005) DNA barcoding: Error rates based on comprehensive sampling. PLoS Biology, 3, 1–10.

https://doi.org/10.1371/journal.pbio.0030422

Nagy, Z.T., Sonet, G., Glaw, F. & Vences, M. (2012) First large-scale DNA barcoding assessment of reptiles in the biodiversity hotspot of Madagascar, based on newly designed COI primers. PLoS ONE, 7, e34506.

http://dx.doi.org/10.1371/journal.pone.0034506.

Pous, P. de, Machado, L., Metallinou, M., Červenka, J., Kratochvíl, L., Paschou, N., Mazuch, T., Šmíd, J., Simó-Riudalbas, M., Sanuy, D. & Carranza, S. (2016) Taxonomy and biogeography of Bunopus spatalurus (Reptilia; Gekkonidae) from the Arabian Peninsula. Journal of Zoological Systematics and Evolutionary Research, 45, 67–81.

http://dx.doi.org/10.1111/jzs.12107.

Rastegar-Pouyani, E., Oraie H., Khosravani, A., Kaboli, M., Mobaraki A., Masoud Yousefi, Behrooz, R., Fakharmanesh, Z. & Wink, M. (2014) A re-evaluation of taxonomic status of Montivipera (Squamata: Viperidae) from Iran using a DNA barcoding approach. Biochemical Systematics and Ecology, 57, 350–356.

https://doi.org/10.1016/j.bse.2014.09.017.

Ronquist, F. & Huelsenbeck, J.P. (2003) MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics, 19, 1572–1574.

https://doi.org/10.1093/bioinformatics/btg180

Savolainen, V., Cowan, R.S., Vogler, A.P., Roderick, G.K. & Lan, R. (2005) Towards writing the encyclopedia of life: an introduction to DNA barcoding. Philosophical Transactions of the Royal Society B, 360, 1805–1811.

https://doi.org/10.1098/rstb.2005.1730

Silvestro, D. & Michalak, I. (2012) RaxmlGUI: a graphical front-end for RAxML. Organisms Diversity & Evolution. 12, 335–337.

https://doi.org/10.1007/s13127-011-0056-0.

Swofford, D.L. (2001) PAUP*, Phylogenetic Analysis Using Parsimony (*and Other Methods), Version 4.0b10. Sinauer Associates, Sunderland, Massachusetts.

Szczerbak, N.N. & Golubev, M.L. (1996) Gecko Fauna of the USSR and Contiguous Regions. Society for the Study of Amphibians and Reptiles, Ithaca, New York. [English edition translated from the Russian by M.L. Golubev & S.A. Malinsky; Leviton, A.E. & Zug, G.R., eds.]

Tamura, K., Stecher, G., Peterson, D., Filipski, A. & Kumar, S. (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Molecular Biology and Evolution, 30, 2725e2729.

https://doi.org/10.1093/molbev/mst197

Vasconcelos, R., Montero-Mendieta, S., Simó-Riudalbas, M., Sindaco, R., Santos, X., Fasola M., Llorente, G., Razzetti, E. & Carranza, S. (2016) Unexpectedly high levels of cryptic diversity uncovered by a complete DNA barcoding of reptiles of the Socotra Archipelago. PLoS ONE, 11 (3), 0149985.

https://doi.org/10.1371/journal.pone.0149985

Uetz, P. & JiríHošek (Eds.) (2016) The Reptile Database. Available from: http://www.reptile-database.org (Accessed 15 Dec. 2017)

Cited by 1 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3