Revalidation of Phalangomyia Dyar & Knab as a subgenus of Culex L. (Diptera: Culicidae) based on morphological and molecular evidence
Author:
GONZÁLEZ CHRISTIAN R.ORCID, REYES-VALENZUELA CAROLINAORCID, ROSSI GUSTAVO C.ORCID, LAURITO MAGDALENA
Abstract
Culex apicinus Philippi, 1865 and its synonyms, Phalangomyia debilis Dyar & Knab, 1914, Cx. escomeli Brèthes, 1920 and Cx. alticola Martini, 1931, were re-examined employing molecular and morphological phylogenetic methods. Archival and fresh specimens were used for morphological diagnosis and to generate fragments of two single-copy nuclear genes (CAD, HB) and one mitochondrial gene (COI). Additional sequences of Cx. apicinus and species of the subgenus Culex of Culex Linnaeus, 1758 and other subgenera of the genus were obtained from GenBank for molecular analysis. Diagnostic morphological characteristics of Cx. apicinus and other species of the subgenus Culex were contrasted. Phylogenetic relationships were generated using DNA sequences of protein coding genes under both Maximum Likelihood and Bayesian approaches. Culex apicinus showed unique morphological traits that allow its classification in a separate subgenus. The subspecific status is supported by its recovery as a monophyletic clade within the genus Culex based on morphological and molecular characters. The synonymous Phalangomyia debilis provides an available subgeneric name. Consequently, Phalangomyia Dyar & Knab, 1914 is resurrected from synonymy with Culex Linnaeus, 1758 as a monobasic subgenus of Culex.
Subject
Animal Science and Zoology,Ecology, Evolution, Behavior and Systematics
Reference39 articles.
1. Lynch, S.E. (2016) Molecular identification of mosquitoes (Diptera: Culicidae) in southeastern Australia. Ecology and Evolution, 6 (9), 3001-3011. https://doi.org/10.1002/ece3.2095
Belkin, J.N. (1968) Mosquito studies (Diptera: Culicidae). IX. The type specimens of New World mosquitoes in European museums. Contributions of the American Entomological Institute, 3 (4), 1-69.
Bourke, B.P., Conn, J.E., de Oliveira, T.M.P., Chaves, L.S.M., Bergo, E.S., Laporta, G.Z. & 2. Sallum, M.A.M. (2018) Exploring malaria vector diversity on the Amazon frontier. Malaria Journal, 17 (1), 342. https://doi.org/10.1186/s12936-018-2483-2
Bourroul, C. (1904) Mosquitos do Brasil. These, Faculdade de Medicina da Bahia. Officina Typographica, Bahia, Consistingof Prefacio pp. i-viii + Novas especies pp. l-6 + 30-32 + Quadro das especies encontradas na Bahia pp. 1-4 and Proposições pp. 1-18. [by Bourroul 3. remainder and bulk of work by Adolpho Lutz]
Bram, R.A. (1967) Classification of Culex subgenus Culex in the New World (Diptera: Culicidae). Proceedings of the United States National Museum, 120 (3557), 1-122.
Brèthes, J. (1920) Description d'un nouveau moustique du Perou. Revista Chilena de Historia Natural, 24, 41-43.
Brower, A.V.Z. & 4. DeSalle, R. (1994) Practical and theoretical considerations for choice of a DNA sequence region in insect molecular systematics, with a short review of published studies using nuclear gene regions. Annals of the Entomological Society of America, 87 (6), 702-716. https://doi.org/10.1093/aesa/87.6.702
Brustolin, M., Talavera, S., Santamaría, C., Rivas, R., Pujol, N., Aranda, C., Marquès, E., Valle, M., Verdún, M., Pagès, N. & 5. Busquets, N. (2016) Culex pipiens and Stegomyia albopicta (= Aedes albopictus) populations as vectors for lineage 1 and 2 West Nile virus in Europe. Medical and Veterinary Entomology, 30 (2), 166-173. https://doi.org/10.1111/mve.12164
Carpintero, D.J. &
|
|