Abstract
Nymphomyiidae from the Russian Far East and bordering territories are revised, including data on taxonomy, redescriptions, and results of DNA barcoding. Nymphomyia orientalis sp. nov. with two subspecies N orientalis orientalis subsp. nov. and N. orientalis makcha subsp. nov. are described and information on biology, ecology and distribution are presented. Key of Nymphomyia species for adult males and females of the world is provided. Three species delimitation approaches (ASAP, mPTP, GMYC) using the mitochondrial gene cytochrome c oxidase I (COI) confirm validity of described taxa as well as available nymphomyiids in GenBank. Morphologically indistinguishable specimens of N. orientalis sp. nov. from Makcha and Polovinka rivers belongs to separate mOTUs with the lowest distances in the genus 1.99% that is the lower interspecific threshold.
Reference35 articles.
1. Astakhov, M.V. (2021) Benthos and syrton composition in two streams of Kunashir Island (Kurile Islands). Vladimir Ya. Levanidov’s Biennial Memorial Meetings, 9, 15–30. [in Russian] https://doi.org/10.25221/levanidov.09.03
2. Bertone, M.A., Courtney, G.W. & Wiegmann, B.M. (2008) Phylogenetics and temporal diversification of the earliest true flies (Insecta: Diptera) based on multiple nuclear genes. Systematic Entomology, 33, 668–687. https://doi.org/10.1111/j.1365-3113.2008.00437.x
3. Courtney, G.W. (1994) Biosystematics of the Nymphomyiidae (Insecta, Diptera): life history, morphology, and phylogenetic relationships. Smithsonian contributions to zoology, 550, 1–39. https://doi.org/10.5479/si.00810282.550
4. Enushenko, I.V. & Makarchenko, E.A. (2019) Discovery of Nymphomyia larval remains (Insecta: Diptera: Nymphomyiidae) in five hundred year-old bottom sediments of Oron lake (Eastern Siberia, Russia). Invertebrate Zoology, 16 (3), 219–225. https://doi.org/10.15298/invertzool.16.3.02
5. Folmer, O., Black, M., Hoeh, W., Lutz, R. & Vrijenhoek, R. (1994) DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology, 3, 294–299.