Abstract
Onnia pakistanica, a novel species, is described from Khyber Pakhtunkhwa, Pakistan. This species is distinguished from its closest kin, O. tibetica, by its unique rusty orange to clay-buff and fawn pileus, larger (5.0–10.1 × 2.5–9.0 μm) basidiospores, broader and fusoidal cystidioles (11.1–16 × 6.5–8.6 μm) and setae with more or less rounded at the tips and broader at the base. The taxonomic status of this species is supported by molecular phylogenetic analysis using ITS and combined ITS and LSU sequences. The presence of O. pakistanica in Pakistan also marks the first genus record from South Asia, adding to the significance of this discovery.