Taxonomic delimitation of endemic Ficus amplocarpa and Ficus dalhousiae Complexes (Moraceae) by DNA barcoding

Author:

MAHIMA KARTHIKEYAN,SUDHAKAR JANA VENKATA,RAMALINGAM SATHISHKUMARORCID

Abstract

Ficus L. species are extensively diversified in India representing North-eastern states, peninsular region and Andaman and Nicobar Islands among which ten species are endemic. Thus closely related species confuses taxonomists and evolutionary researchers. Our study aims to distinguish the highly endangered closely allied species of Ficus genus with persistent variation. The first study complex includes Ficus dalhousiae and Ficus anamalayana and the second complex includes Ficus amplocarpa, Ficus guttata and Ficus laevis. The species in each complex groups are hard to distinguish because of their highly similar morphological characters. To resolve the taxonomical disputes, the nuclear loci ITS2 and chloroplast loci trnH-psbA were used to reconstruct the phylogenetic relationship to understand the species boundaries. In our results, intra-specific divergence is not found in the two markers tested however, the total inter-specific divergence between F. dalhousiae and F. anamalayana is found to be 6.5% and in the case of F. amplocarpa and F. guttata is to be 8.5%. The highest divergence is observed in trnH-psbA (0.991 ± 0.713) followed by ITS2 (0.149 ± 0.064). Bayesian analysis showed a clear segregation between F. amplocarpa and F. guttata suggesting that they could be independent species not a variety of F. laevis. Similarly, bayesian analysis of F. dalhousiae and F. anamalayana results support that they can be treated as different species. Thereby, our study suggests that species within taxonomically complex group can be resolved by DNA barcoding.

Publisher

Magnolia Press

Subject

Plant Science,Ecology, Evolution, Behavior and Systematics

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3