Development of SSR markers identification system for Carex L. based on RAD sequencing

Abstract

In this study, we designed and evaluated an efficient set of simple sequence repeat (SSR) markers from restriction site-associated DNA (RAD) sequencing data of four widely utilized Carex L. accession across Northern China, especially in Beijing. Based on their genomic sequencing data, we developed 400 SSR markers and evaluated their amplification specificities among which a total of 17 SSR markers were identified as a core set of molecular markers for efficient assessment of diverse Carex L. accessions. Using this molecular identification system, we classified 26 Carex L. accessions into 3 genetically distinct groups. The establishment of a molecular identification system based on a core set of 17 SSR markers provides an essential basis for evaluating genetic relationships among Carex L. accessions along with a wealth of genetic resources for marker-assisted selection and breeding of Carex L..