Biological mechanisms underlying priming of vascular plant material in the presence of diatoms

Author:

Bonin P1,Portas A1,Hardy J1,Guasco S1,Bianchi TS2,Ward ND34,Rontani JF1

Affiliation:

1. Aix Marseille Univ, Université de Toulon, CNRS, IRD, MIO UM 110, 13288 Marseille, France

2. Department of Geological Sciences, Box 112120, University of Florida, Gainesville, FL 32611-2120, USA

3. Marine and Coastal Research Laboratory, Pacific Northwest National Laboratory, 1529 W Sequim Bay Rd, Sequim, WA 98382, USA

4. School of Oceanography, University of Washington, Box 355351, Seattle, WA 98195, USA

Abstract

Priming effects that stimulate increased degradation of refractory organic matter by microorganisms following fresh organic matter input is a well-known phenomenon in terrestrial environments but remains controversial in marine environments. We used a combination of chemical (gas chromatography-EI quadrupole time of flight mass spectrometry) and molecular biology (DNA stable-isotope probing [DNA-SIP]) methods to trace the fate of terrestrially derived particulate organic matter (TPOM) and the response of the marine microbial community to fresh organic matter inputs. We tested the potential for priming effects among a mixture of marine and terrestrial microbial assemblages, amended either with only 13C-labelled TPOM (13C-Avena sativa) or with an addition of 12C-Skeletonema costatum (a marine diatom) as a labile co-substrate within the range of diatom concentrations found in estuaries. We monitored 13C-labelled TPOM lipid tracers (long-chain fatty acids, n-alkan-1-ols, phytol, sitosterol, β-amyrin and components of cutins) throughout a 42 d incubation experiment. Comparisons with controls carried out without diatom addition showed faster decay of phytol, n-alkan-1-ols, and components of cuticular waxes in the presence of diatoms, while fatty acids and sitosterol were unaffected. Bacteria belonging to the Bacteroidota phylum (mainly Flavobacteria and Cytophaga) were the dominant microbes involved in priming-induced TPOM degradation in the incubation treatments. Sphingomonadales and Rhizobiales, capable of lignin and hemicellulose degradation, also contributed to the degradation of TPOM but did not seem to contribute to priming effects related to increased diatom abundance. These lab-based results demonstrate direct evidence that priming of TPOM occurred selectively via a consortium of microbes.

Publisher

Inter-Research Science Center

Subject

Aquatic Science,Ecology, Evolution, Behavior and Systematics

Cited by 2 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

1. Mangrove peat and algae leachates elicit rapid and contrasting molecular and microbial responses in coastal waters;Communications Earth & Environment;2023-10-14

2. Organic Carbon Cycling and Transformation;Reference Module in Earth Systems and Environmental Sciences;2023

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