Treatment of Phenol-Containing Wastewater by the Bacterial Strain Serratia marcescens MT9

Abstract

The features of the phenol and 2,4-dichlorophenol-degrading strain S. marcescens MT9, isolated from the soil of the Ufa (Republic of Bashkortostan) a major producer of organic chemistry products in Russia, are described. Identification of the strain was performed according to the cultural, morphological, physiological, biochemical, morphometric features, as well as the results of the comparative analysis of the 16S rRNA gene sequence. The growth of S. marcescens MT9 in the batch culture with phenol and 2,4-dichlorophenol as sole source of carbon and energy in concentration 100 mg/l was studied. It was established that the phenol and 2,4-dichlorophenol content in the culture liquid was reduced to the 6th day by 82 % and to the 8ths day by 65 % from the control, respectively. The possibility of using S. marcescens MT9 for phenol and 2,4-dichlorophenol utilization in industrial wastewater of petrochemical production and production of tanning extracts has been revealed. The level of wastewater treatment using strain S. marcescens MT9 was 89,3–99,6 %.