Green synthesis, Characterization of Silver Nanoparticles and Antimicrobial Activity using Rauvolfia tetraphylla L.

Abstract

Rauvolfia tetraphylla L is one among the traditional plants that has more therapeutic effects and numerous beneficial components, few of them were majorly used as a medicine for snake bites. This medicinal plant has immense pharmacological properties that could aid in formulating a drug for treating diseases. This was the major motivation for the study on Rauvolfia tetraphylla. The collected fresh samples were washed and shade dried for a week, then made into fine powder and ethyl acetate based crude extract was produced. The phytochemical and functional group were analysed, protein estimation was done by Lowry’s method. Further anti-oxidant properties were detected by DPPH. Disk diffusion method was used to measure its antibacterial activity. GC-MS analysis was done to find out the compounds in the extract. Silver nanoparticles were synthesized. Characterization of silver nanoparticles was done by FTIR, UV spectrometry and SEM. The preliminary phytochemical analysis shows presence of flavonoids, terpenoids and phenolic compounds. The antibacterial assay shows zone formation against the pathogen used. Also the ethyl acetate extract shows more than 20 compounds through GC-MS. The silver nanoparticles synthesis was confirmed by UV spectra around 440nm and characterized by FTIR and SEM. The ethyl acetate extract of leaves of Rauvolfia tetraphylla (L) has shown seemingly great pharmacological properties.