Biomimetic chitosan against bioinspired nanohydroxyapatite for repairing enamel surfaces

Abstract

In this study, chitosan was employed as a novel biomimetic mineralization model to repair damaged enamel to compare its performance with that of bioinspired zinc-doped nanohydroxyapatite. Fifty human premolar tooth slices were prepared, and artificial caries lesions were induced to produce demineralized enamel surfaces. The etched slices were randomly divided into two groups: a chitosan-hydrogel-treated group and a zinc-doped nanohydroxyapatite-treated group. In vitro assessment using energy-dispersive X-ray analysis, X-ray diffraction analysis and scanning electron microscopy was conducted at the baseline, demineralization and remineralization stages. Baseline results were matched with those for normal enamel; a marked reduction in the calcium (Ca)/phosphorus (P) ratio to 1·12 and the lack of the characteristic hydroxyapatite diffraction peaks were detected for demineralized enamel. The remineralization stage revealed evident recovery of the mineral contents (the calcium/phosphorus ratio was 1·61 for the chitosan-treated group and 1·58 for the bioinspired-nanohydroxyapatite-treated one), with apparent distinctive X-ray diffraction patterns of hydroxyapatite in both groups. Scanning electron microscopic analysis showed the absence of etched enamel porosity, with the formation of a newly formed rod-like apatite layer, similar to natural enamel, which extended over the treated enamel surfaces of both groups. Chitosan hydrogel is recommended as a biomimetic mineralization smart system for repairing demineralized carious enamel.