Hydroxyapatite/silk fibroin composite biomimetic scaffold for dental pulp repair

Author:

Zhang Wu1,Liu Haixia2,Yang Wei3,Liu Chang4,Xie Miaomiao5,Guo Rui3ORCID,Liang Jianqiang6,Ye Zhongtai5,Xu Hao7

Affiliation:

1. The First Affiliated Hospital of Jinan University, Guangzhou, China; School of Stomatology, Jinan University, Guangzhou, China

2. Guangzhou Nansha District Maternal and Child Health Care Hospital, Guangzhou, China

3. Key Laboratory of Biomaterials of Guangdong Higher Education Institutes, Department of Biomedical Engineering, Jinan University, Guangzhou, China

4. School of Stomatology, Jinan University, Guangzhou, China

5. Department of Stomatology, People’s Hospital of Baoan District, Shenzhen, China

6. Guangzhou Haizhu District Stomatological Hospital, Guangzhou, China

7. The First Affiliated Hospital of Jinan University, Guangzhou, China

Abstract

Dental pulp repair is a difficult clinical problem. In the present study, the authors aimed to mimic the extracellular matrix of dental pulp tissue structurally and compositionally. Nanofibrous silk fibroin (SF) scaffolds containing hydroxyapatite (HAp) nanoparticles were fabricated by using the freeze-drying approach. Rod-shaped HAp was successfully embedded in the composite scaffold, the diameter of which was about 100–200 nm as shown by transmission electron microscopy analysis. The three-dimensional microstructure of the composite scaffold prepared in various ratios of HAp to SF was observed by scanning electron microscopy and the pore size of the optimal scaffold was about 30–120 μm. Meanwhile, the hemocompatibility of the composite scaffolds was evaluated based on their impact on the clotting function by way of activated partial thromboplastin time, prothrombin time and thromboelastographic assays. The scaffolds possessed a low hemolysis rate of red blood cells. Furthermore, cell culture tests using dental pulp stem cells found that the scaffolds had good biocompatibility. There biomimetic HAp/SF composite scaffolds may serve as a promising biomaterial for dental pulp repair.

Publisher

Thomas Telford Ltd.

Subject

General Engineering,Biomaterials

Cited by 11 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

1. Naturally-Derived Biomaterials for Oral and Dental Tissue Engineering;Functional Bio-based Materials for Regenerative Medicine: From Bench to Bedside (Part 2);2024-01-29

2. Role and importance of hydroxyapatite in the healthcare sector;Industrial Applications of Nanoceramics;2024

3. Advances in scaffolds used for pulp–dentine complex tissue engineering: A narrative review;International Endodontic Journal;2022-09-16

4. Postcare for Repairing Nerve and Tendon Injury Based on Biomimetic Nano-Parallel Material Composite Protein;Advances in Materials Science and Engineering;2022-07-20

5. Extraction of Silk Fibroin with Several Sericin Removal Processes and its Importance in Tissue Engineering: A Review;Journal of Polymers and the Environment;2022-01-20

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