A method for visualizing of reproductive organs in immature small mammals using Soricidae (Insectivora) and Cricetidae (Rodentia) as an example-Reference-Cited by-同舟云学术

A method for visualizing of reproductive organs in immature small mammals using Soricidae (Insectivora) and Cricetidae (Rodentia) as an example

Published:2022-09-25 Issue:3 Volume:326 Page:164-171
ISSN:0206-0477
Container-title:Proceedings of the Zoological Institute RAS
language:ru
Short-container-title:Proceedings ZIN

Author:

Saburova L.Ya.¹

Affiliation:

1. N. Laverov Federal Center for Integrated Arctic Research of the Ural Branch of the Russian Academy of Sciences, 163069, Severnoy Dviny Emb. 23, Arkhangelsk, Russia

Abstract

The article is devoted to determination of sex of juveniles of small mammals. The definition of which is the most time-consuming part of anatomical research. The method of visualizing the sex of young animals by reproductive organs using 3% hydrogen peroxide (H2O2) and 6% apple cider vinegar (CH3COOH) is used. Earlier in 2020, this method was successfully used on immature shrews (Sorex). The results of the study served as an impetus for the continuation of the study on juveniles of the hamster family (Cricetidae Fischer, 1817) and repeated testing on young individuals of the shrew family (Soricidae Fischer, 1814). The collection of material was carried out in 2021 in the central part of the White Sea-Kuloy Plateau. A total of 475 small mammals from three families were studied (Cricetidae – 291, Soricidae – 183, birch mice Sminthidae Brandt, 1855 – 1). Anatomical examination of biological material showed that 24% (n = 114) of young animals turned out to have indeterminate sexual characteristics. These juvenile individuals belonged to the families of shrews (15.8%) and hamsters (8.2%). As a result of the method, gender differences were found in 92.1% of juvenile individuals. During the reaction of hydrogen peroxide with blood catalase, in 35.2% of males (of the identified individuals), the spermatic ducts were elevated, and in 51.4% of them, seminal ducts and testes were discolored. In 43.8% of females, the horns and the body of the uterus increased in size by 2 times, and it turned white in 48.6%. In 7.9% of specimens, the sex has not been set due to postmortem damage to internal organs. The study proves that the method of visualizing sex by reproductive organs is effective for immature animals of Cricetidae and Soricidae. The method makes it possible to reduce the number of unrecorded juveniles and to obtain reliable information about the sex and age structure of the population.

Publisher

Zoological Institute of the Russian Academy of Sciences

Subject

Insect Science,Animal Science and Zoology,Aquatic Science,Ecology, Evolution, Behavior and Systematics

Reference21 articles.

1. Anikanova V.S., Bugmyrin S.V. and Ieshko E.P. 2007. Methods for collection and study of helminths in small mammals. Karelian Research Centre of RAS, Petrozavodsk, 145 p. [In Russian].

2. Baldzhi Yu.A., Majkanov B.S., Zhubatkanova A.Zh. and Adil'bekov Zh.Sh. 2015. Development of express method for meat quality determination. Aktual'nye problemy i dostizheniya v sel'skohozyajstvennyh naukah. Sbornik nauchnyh trudov po itogam mezhdunarodnoj nauchno-prakticheskoj konferencii. V. 2. (April 7, 2015, Samara). Innovative center for the development of education and science, Samara: 34–37. [In Russian].

3. Bobretsov A.V. 2016. Population ecology of small mammals of plain and mountain landscapes of North-East of the European part of Russia. KMK Scientific Press, Moscow, 31 p. [In Russian].

4. Geodakyan V.A. 2012. Two Sexes. Why? The Evolutionary Theory of Sex. Moscow, 246 p. [In Russian]. https://batrachos.com/sites/default/files/pictures/Books/geodakyan_2012_dva_pola_zachem_i_pochemu_evolyucionnaya_teoriya_pola.pdf

5. Karaseva E.V., Telicyna A.Yu. and Zhigal'skij O.A. 2008. The methods of studying rodents in the wild nature. Publishing house LKI, Moscow, 416 p. [In Russian].