A ONE-WAVELENGTH, TWO-AREA METHOD IN MICROSPECTROPHOTOMETRY FOR PURE AMPLITUDE OBJECTS

Abstract

A one-wavelength method for nuclei in smears is tested and discussed. It eliminates need for visual estimates of areas and measurements to check for possible slight variations in absorption spectrum which would vitiate applicability of the two-wavelength method. Extinction ( E1) is obtained from an intranuclear plug measurement; area of absorbing material is computed as a function of ratios between "one minus transmittances", one intranuclear, the second including in addition "free" or "non-absorbing" areas around it. If T1 represents intranuclear transmittance, T2 total transmittance (nucleus plus free area), A1 the area of the nucleus and A2 the area from which T2 was obtained (absorbing plus nonabsorbing media), total extinction times area is computed as [See equation in the PDF file]