Expression Profiling of the Ephrin (EFN) and Eph Receptor (EPH) Family of Genes in Atherosclerosis-Related Human Cells

Abstract

Ephrin B1 and its cognate receptor, Eph receptor B2, key regulators of embryogenesis, are expressed in human atherosclerotic plaque and inhibit adult human monocyte chemotaxis. Few data exist, however, regarding the gene expression profiles of the ephrin ( EFN) and Eph receptor ( EPH) family of genes in atherosclerosis-related human cells. Gene expression profiles were determined of all 21 members of this gene family in atherosclerosis-related cells by reverse transcription—polymerase chain reaction analysis. The following 17 members were detected in adult human peripheral blood monocytes: EFNA1 and EFNA3 – EFNA5 (coding for ephrins A1 and A3 – A5); EPHA1, EPHA2, EPHA4 – EPHA6 and EPHA8 (coding for Eph receptors A1, A2, A4 – A6 and A8); EFNB1 and EFNB2 (coding for ephrins B1 and B2); and EPHB1 – EPHB4 and EPHB6 (coding for Eph receptors B1 – B4 and B6). THP-1 monocytic cells, Jurkat T cells and adult arterial endothelial cells also expressed multiple EFN and EPH genes. These results indicate that a wide variety of ephrins and Eph receptors might affect monocyte chemotaxis, contributing to the development of atherosclerosis. Their pathological significance requires further study.