Histological Assessment of the Effect of α-Tocopherol on Fracture Healing in Rabbits

Abstract

To investigate the effect of α-tocopherol (vitamin E) on fracture healing in rabbits, two groups of 10 rabbits were either injected with α-tocopherol (treated) or untreated (controls). The right femurs of both groups were fractured, and the treated group were injected intramuscularly with 20 mg/kg α-tocopherol daily for 5 days starting on the day of fracture. After 21 days, histological sections of the fractured region were examined and scored. Fracture healing had progressed further in the α-tocopherol group than in the control group. A statistically significant difference between the histological grading of fracture healing in the two groups was found. This difference may result from an antioxidant (α-tocopherol) effect on free oxygen radicals in the fracture area. We conclude that α-tocopherol may affect fracture healing favourably and might be useful as a therapeutic agent in clinical fracture management.