Mutagenicity of Helicobacter Pylori in the Ames Test Using Salmonella Typhimurium TA100

Abstract

Infection with Helicobacter pylori has been recognized as a risk factor for gastric cancer, although it is still unclear whether strains of this organism behave as carcinogens. We have studied 30 H. pylori clinical isolates from 15 patients with gastric cancer and 15 patients with other gastroduodenal diseases. Bacterial pellet and supernatant fluid of broth cultures were tested for mutagenicity by means of the Ames test using the pre-incubation technique. The average Ames ratio was 1.111 in the bacterial pellet and 1.312 in the supernatant of strains from the gastric-cancer group, and 0.858 and 0.950 in those from the non-gastric-cancer group, respectively. The strains from the gastric-cancer group had a significantly higher ratio than those from the non-gastric-cancer group, although all results were below the cut-off ratio of the Ames test, and were thus regarded as having no mutagenicity. The Ames ratio of the supernatant was higher than that of the bacterial pellet in both groups. The results indicate that all of the H. pylori strains tested revealed no mutagenicity, but the difference in Ames ratio between strains might reflect differences in genotoxicity between the strains.