Serum Levels of Tumor Necrosis Factor Alpha Correlate with Response to Neoadjuvant Chemotherapy in Locally Advanced Breast Cancer

Abstract

It has been shown that serum levels of tumor necrosis factor alpha (TNF-α) are increased in breast cancer patients. There are few data available on the reduction of serum levels of this cytokine following chemotherapy. The aim of this study was to determine the effect of neoadjuvant chemotherapy on serum concentrations of TNF-α and the relation to response rates in locally advanced breast cancer. Twenty consecutive patients with non-inflammatory stage III-B breast cancer achieving a partial or complete clinical response to three courses of neoadjuvant chemotherapy followed by modified radical mastectomy were prospectively included in the study and evaluated. Sera were collected before the start and after the termination of chemotherapy. Serum concentrations of TNF-α were measured by an ELISA method. The pathological response rates were also evaluated and recorded. The control group consisted of 12 healthy age-matched women. The mean pre-treatment TNF-α value of breast cancer patients was 15.9±0.9 pg/mL while it was 5.8±1.7 pg/mL in the control group; the difference was statistically significant (p<0.0001). The serum levels of TNF-α were markedly decreased in patients with partial and complete responses compared to pre-treatment values (p<0.0001). There was also a difference in TNF-α levels in patients with partial vs complete responses (p<0.0001). The relative change between pre- and post-treatment values correlated significantly with the type of response (p=0.004). These results suggest that the serum concentration of TNF-α can be an indicator of response and could be used in clinical decision-making for patients with locally advanced breast cancer.