The effect of LDHs nanoparticles on the cellular behavior of stem cell-laden 3D-bioprinted scaffold

Abstract

Three-dimensional (3D)-bioprinting as an emerging approach for tissue engineering possesses the promise to create highly mimicked organs or tissues by using computer-aided design. For biomedical applications in tissue engineering in our previous work, we developed an optimized nanocomposite bioink based on methylacrylated gelatin (GelMA), methylacrylated chitosan (ChitMA), and double-layered hydroxide (LDHs) nanoparticles by using 3D-bioprinting technology. Herein, we used the previous formulation to fabricate human bone marrow mesenchymal stem cells (hBMMSCs)-laden nanocomposite bioinks. The effect of LDHs nanoparticles on the cellular behaviors of the encapsulated-hBMMSCs in the scaffolds was evaluated for the first time. Live/Dead, PrestoBlue, and DAPI/Actin analysis were carried out to assess the cell viability, proliferation rate, and cellular morphology of encapsulated hBMMSCs within the scaffolds. In addition, osteogenic differentiation studies were performed culturing the scaffolds for up to 21 days. Results show that LDHs nanoparticles in the GelMA/ChitMA scaffold formulation increased the viability of hBMMSCs, did not cause any adverse effect on the proliferation rate, cell morphology of the hBMMSCs, and increased the Runx2 protein expression of the encapsulated-hBMMSCs in the scaffolds. This study progresses the LDHs containing nanocomposite bioink for cell printing applications in tissue engineering.