Compatibility of Embryonic Stem Cells with Biomaterials

Author:

Handschel Jörg1,Berr Karin1,Depprich Rita2,Naujoks Christian1,Kübler Norbert R.1,Meyer Ulrich1,Ommerborn Michelle3,Lammers Lydia4

Affiliation:

1. Department for Cranio- and Maxillofacial Surgery Heinrich-Heine-Universität, Moorenstr. 5, 40225 Düsseldorf, Germany

2. Department for Cranio- and Maxillofacial Surgery Heinrich-Heine-Universität, Moorenstr. 5, 40225 Düsseldorf, Germany,

3. Department for Operative and Preventive Dentistry and Endodontics, Heinrich-Heine-University Düsseldorf Moorenstr. 5, 40225 Düsseldorf, Germany

4. Department for Cranio- and Maxillofacial Surgery Westfälische-Wilhelms-Universität, Waldeyerstr. 30 48149 Münster, Germany

Abstract

Periodontal bone defects and atrophy of the jaws in an aging population are of special concern. Tissue engineering using embryonic stem cells (ESCs) and biomaterials may offer new therapeutic options. The purpose of this study is to evaluate the compatibility of ESCs with biomaterials and the influence of biomaterials on the osteogenic gene expression profile. Therefore, ESCs are cultured with various biomaterials. The cytocompatibility of murine ESCs is measured regarding the proliferation of the cells on the materials by CyQUANT ® assay, the morphology by scanning electron microscopy, and the influence on the gene expression by real time PCR. The results show that insoluble collagenous bone matrix, followed by β-tricalciumphosphate, is most suitable for bone tissue engineering regarding cell proliferation, and phenotype. The gene expression analysis indicates that biomaterials do influence the gene expression of ESCs. Our results provide new insight into the cytocompatibility of ESCs on different scaffolds.

Publisher

SAGE Publications

Subject

Biomedical Engineering,Biomaterials

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