Gelatin gel as a carrier of platelet-derived growth factors

Abstract

Currently, patient’s own growth factors from platelet-rich plasma and platelet-rich fibrin have been clinically used for repair and regeneration of defective tissues. In platelet-rich plasma and platelet-rich fibrin, fibrin gel is formed from blood fibrinogen and functions as a carrier for growth factors. In this study, the growth factors were extracted from the platelet-rich fibrin and incorporated into a gelatin gel by mixing the platelet-rich fibrin extract and gelatin solution before cross-linking with glutaraldehyde. About 70% of TGF-β1 was found to be released in vitro from the gelatin gel containing the platelet-rich fibrin extract into phosphate-buffered saline (–) in 7 days. The gelatin gel containing basic fibroblast growth factor, prepared as a comparison, showed a similar release profile. The gelatin gels were slowly degraded with time after subcutaneous implantation on the back of rats, and the gel containing the platelet-rich fibrin extract strongly induced neovascularization and granulation tissue formation around the implantation site compared to the gel only and the gel containing basic fibroblast growth factor, platelet-rich plasma, or platelet-rich fibrin. The gelatin gel containing the platelet-rich fibrin extract was attempted as wound dressing on a full-thickness skin defect model. After 2 weeks of application, the gel was found to be more effective in acceleration of wound healing than the commonly used platelet-rich plasma.