A Short-Wavelength Raman Optical Activity Spectrometer with Laser Source at 457 nm for the Characterization of Chiral Molecules

Abstract

Developing a high-sensitivity Raman optical activity (ROA) spectrometer has been regarded as one of the great challenges in chiral science and technology. Herein, we report our recent progress on the development of a short-wavelength ROA (sw-ROA) spectrometer with the excitation line at 457 nm, which shows obviously improved signal-to-noise (S/N) ratio compared with the currently available 532 nm ROA spectrometer. This could be ascribed to the fifth-power of frequency dependence for ROA intensity together with the potential advantage of avoiding fluorescence for most molecules. The required laser power at the sample for being able to obtain a reliable ROA spectrum is less than 150 milliwatts (mW) for most samples. In the case of neat S-α-pinene sample, the ROA signal can be acquired with the laser power at sample as low as 5 mW with the total exposure time of 5 min. The concentration of S-α-pinene sample can be reduced to 10% (v/v) by diluting with ethanol. These results demonstrate the great potential of sw-ROA (457 nm) working with decreased laser power, shortened acquisition time, and lower sample concentration. The applicability of sw-ROA (457 nm) has also been demonstrated by measuring representative chiral samples, including carbohydrates, amino acids, protein in aqueous solution, and chiral organic molecule in organic solvents.