Rhodamine 6G–Anionic Surfactant System Modified by Triton X-100 for Fluorescence Determination of Albumin

Abstract

The influence of sodium hexadecyl sulfate on the nature of the fluorescence spectra of rhodamine 6G in an aqueous solution and a solution of nonionic Triton X-100 was investigated. The change in the nature of the emission spectra is explained by the formation of hydrophobic stoichiometric and sub-stoichiometric reagent-surfactant associates. Stabilization of the colloid-chemical state and reduction of the total turbidity of rhodamine 6G–anionic surfactant associate solutions with the addition of nonionic surfactant as a modifier were registered. The method of modification of the rhodamine 6G–sodium hexadecyl sulfate system with a nonionic surfactant was used in the development of conditions for the fluorescence determination of protein substances in physiological solutions. The concentration conditions for the use of the modified reagent system rhodamine 6G–anionic surfactant–nonionic surfactant for the fluorescence determination of albumin in urine were optimized.