Development of a New Kinetic Spectrophotometric Method for the Determination of Chromium with an Optical Probe

Abstract

The aim of this work is the development, optimization, and validation of a new spectrophotometric kinetic method for the determination of dissolved chromium species in water samples with the use of the polymethine dye Astra Phloxine FF. The progress of the chemical reaction was simple, effective, and precisely monitored from the start of the reaction using an optical probe. The method is based on the impact of Cr(VI) concentration on the rate of decrease in the Astra Phloxine FF concentration. The experimental data were evaluated using four experimental data analysis methods, namely with the initial rate method, the average rate method, the fixed time method, and the absorption peak volume change method. Under the optimal reaction conditions, the best results were achieved using the method of the average rate constant for evaluating the experimental data. Using this data evaluation method for the determination of Cr(VI), the LoD was found to be 1.87 µg L–1 and RSD ( n = 6; 0.2 mg L–1 Cr) 3.59%. The presented work was used for the determination of chromium in model samples—CRM material and tap and waste water—and with the calibration line method and the standard additions method.