Use of Sodium Salicylate as a Blocking Agent for Cortisol-Binding-Globulin in a Radioimmunoassay for Cortisol on Unextracted Plasma-Reference-Cited by-同舟云学术

Use of Sodium Salicylate as a Blocking Agent for Cortisol-Binding-Globulin in a Radioimmunoassay for Cortisol on Unextracted Plasma

Published:1979-01 Issue:1-6 Volume:16 Page:209-212
ISSN:0004-5632
Container-title:Annals of Clinical Biochemistry: International Journal of Laboratory Medicine
language:en
Short-container-title:Ann Clin Biochem

Author:

Kane J. W.¹

Affiliation:

1. Department of Chemical Pathology, The University Hospital of South Manchester, Withington Hospital, Manchester, M20 8LR

Abstract

This report describes investigations into the use of sodium salicylate as a cortisol-binding-globulin blocking agent and the subsequent development of a radioimmunoassay for cortisol on unextracted plasma. Cortisol antiserum was raised against a cortisol 3–0-(carboxymethyl) oxime-bovine serum albumin conjugate. A 125I-labelled cortisol-tyrosine methyl ester conjugate was also prepared for use in the assay. The radioimmunoassay developed involved no pre-treatment or extraction of the samples before analysis and was extremely simple to perform. Comparison with another radioimmunoassay for cortisol and with the Mattingly fluorimetric assay gave good correlation.

Publisher

SAGE Publications

Subject

Clinical Biochemistry,General Medicine

Link

http://journals.sagepub.com/doi/pdf/10.1177/000456327901600149

Reference13 articles.

1. Assessment of a Simple Competitive Protein-Binding Technique for Plasma Cortisol Assay

2. Improved Radioimmunoassay of Plasma Cortisol

3. Single-Antibody Technique for Radioimmunoassay of Cortisol in Unextracted Serum or Plasma

4. Comparison of a Fluorimetric Method and a Competitive Protein Binding Assay Kit for the Determination of Plasma Hydroxycorticosteroids

5. An 125I-labeled cortisol radioimmunoassay in which serum binding protein are enzymatically denatured.

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