Measurement of Urinary Amino Acids Using High-Performance Liquid Chromatography Equipped with a Strong Cation Exchange Resin Pre-Column-Reference-Cited by-同舟云学术

Measurement of Urinary Amino Acids Using High-Performance Liquid Chromatography Equipped with a Strong Cation Exchange Resin Pre-Column

Published:1999-03 Issue:2 Volume:36 Page:202-206
ISSN:0004-5632
Container-title:Annals of Clinical Biochemistry: International Journal of Laboratory Medicine
language:en
Short-container-title:Ann Clin Biochem

Author:

Hara Katsuko¹,Hijikata Yasuyo²,Hiraoka Etsuko¹,Ogawa Noboru¹,Takahashi Hakuo³

Affiliation:

1. Department of Clinical Laboratory, Kansai Medical University, Fumizono-cho 10-15, Moriguchi City, Osaka 570-8507, Japan

2. Toyodo-Hijikata Clinic, Kansai Medical University, Fumizono-cho 10-15, Moriguchi City, Osaka 570-8507, Japan

3. Department of Clinical Sciences and Laboratory Medicine, Kansai Medical University, Fumizono-cho 10-15, Moriguchi City, Osaka 570-8507, Japan

Abstract

We determined the urinary amino acid concentrations by high-performance liquid chromatography (HPLC) using an eluent buffer which had been processed through a column packed with strong cation-exchange resin. With this column, ghost peaks resolved or much reduced in size and shifted to a post-arginine position. A clearer separation of peaks of urinary amino acids and ammonia was obtained. This could be due to the elimination of O-phthalaldehyde reactive substances in the eluent by the resin column. This method does not require a separate step to remove ammonia, unlike most of the methods currently used, and thus saves time.

Publisher

SAGE Publications

Subject

Clinical Biochemistry,General Medicine

Link

http://journals.sagepub.com/doi/pdf/10.1177/000456329903600211

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1. Chapter 14 Chromatography of amino acids and peptides;Journal of Chromatography Library;2004

2. Year-Long Validation Study and Reference Values for Urinary Amino Acids Using a Reversed-Phase HPLC Method;Clinical Chemistry;2001-03-01

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