A Highly Sensitive Assay for Quantitation of Apolipoprotein B48 Using an Antibody to Human Apolipoprotein B and Enhanced Chemiluminescence-Reference-Cited by-同舟云学术

A Highly Sensitive Assay for Quantitation of Apolipoprotein B48 Using an Antibody to Human Apolipoprotein B and Enhanced Chemiluminescence

Published:1997-03 Issue:2 Volume:34 Page:185-189
ISSN:0004-5632
Container-title:Annals of Clinical Biochemistry: International Journal of Laboratory Medicine
language:en
Short-container-title:Ann Clin Biochem

Author:

Smith Darrin¹,Proctor Spencer D¹,Mamo John C L¹

Affiliation:

1. Departments of Medicine and Physiology, University of Western Australia

Abstract

We describe a method for the rapid quantification of serum apolipoprotein B48 using a commercially available anti-apolipoprotein (apo)B antiserum and compare it to analytical sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) with coomassie blue R250 staining. The method described here eliminates the need for de-lipidation of samples and only requires a one-step overnight ultracentrifugation. Western-blotting and enhanced chemiluminescence (ECL) visualization of proteins was approximately 10 times more sensitive than coomassie staining and generally took no longer to complete than staining/destaining of SDS-PAGE gels. The sensitivity of the antiserum/ECL technique enabled quantitation of fasting apolipoprotein B48 which could not be resolved by SDS-PAGE and Coomassie staining.

Publisher

SAGE Publications

Subject

Clinical Biochemistry,General Medicine

Link

http://journals.sagepub.com/doi/pdf/10.1177/000456329703400210

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