A Reassessment of 8-Anilino-1-Naphthalene Sulphonic Acid as a Thyroxine Binding Inhibitor in the Radioimmunoassay of Thyroxine-Reference-Cited by-同舟云学术

A Reassessment of 8-Anilino-1-Naphthalene Sulphonic Acid as a Thyroxine Binding Inhibitor in the Radioimmunoassay of Thyroxine

Published:1977-01 Issue:1-6 Volume:14 Page:330-334
ISSN:0004-5632
Container-title:Annals of Clinical Biochemistry: International Journal of Laboratory Medicine
language:en
Short-container-title:Ann Clin Biochem

Author:

Evans S. E.¹,Burr W. A.¹,Hogan T. C.¹

Affiliation:

1. Departments of Clinical Chemistry and Medicine, Queen Elizabeth Hospital, Edgbaston, Birmingham B15 2TH

Abstract

The effectiveness of 8-anilino-1-naphthalene sulphonic acid (ANS) in the radioimmunoassay (RIA) of thyroxine (T4) as an inhibitor of the binding of T4 to serum T4-binding proteins is assessed. The optimum ANS concentration is dependent upon the antiserum and the method used for separating free and bound T4. If T4 binding to serum proteins is not completely inhibited, resin separation methods may yield low values, while polyethylene glycol and double-antibody methods may produce high values for T4 concentration. Even with optimum ANS concentration gross errors in measurement of T4 may occur in patients with high thyroxine-binding globulin (TBG) concentrations.

Publisher

SAGE Publications

Subject

Clinical Biochemistry,General Medicine

Link

http://journals.sagepub.com/doi/pdf/10.1177/000456327701400186

Reference13 articles.

1. Preparation of a monospecific antiserum to thyroxine binding globulin for its quantitation by rocket immunoelectrophoresis

2. T4 RADIOIMMUNOASSAY AND THYROXINE-BINDING GLOBULIN CONCENTRATION

3. A Radioimmunoassay for Measurement of Thyroxine in Unextracted Serum*

4. The apparent ionization constants of the phenolic hydroxyl groups of thyroxine and related compounds

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