Flow Cell Modification of a Filter Fluorimeter Producing Improved Throughput and Precision

Abstract

A modification of a Corning-EEL 244 filter fluorimeter to take a Hellma fluoronephelometric flow cell is described, and the characteristics of a standard flow cell and the Hellma flow cell are compared by the use of a continuous flow fluorimetric calcein—calcium method. The study shows that by this modification a considerable improvement in precision, carryover, and throughput is possible. The kinetically better Hellma flow cell enables speeds in excess of 70 per hour to be achieved with carryover and precision better than that obtained at 40 per hour with the standard flow cell. Curve regeneration of signals using the Hellma flow cell can also be carried out, enabling a further increase in analytical rate to be obtained.