A Spectrophotometric Assay of Phosphoribosyl Pyrophosphate Synthetase-Reference-Cited by-同舟云学术

A Spectrophotometric Assay of Phosphoribosyl Pyrophosphate Synthetase

Published:1984-09 Issue:5 Volume:21 Page:366-371
ISSN:0004-5632
Container-title:Annals of Clinical Biochemistry: International Journal of Laboratory Medicine
language:en
Short-container-title:Ann Clin Biochem

Author:

Braven J¹,Hardwell T R²,Seddon R³,Whittaker Mary⁴

Affiliation:

1. Department of Environmental Science, Plymouth Polytechnic, Plymouth, UK

2. Department of Chemical Pathology, Torbay Hospital, Torquay, UK

3. Department of Clinical Chemistry, Barnstaple Hospital, Barnstaple, UK

4. Department of Chemistry, University of Exeter, Exeter, UK

Abstract

A simple spectrophotometric assay of phosphoribosyl pyrophosphate synthetase from human erythrocytes is described. The kinetic properties of the enzyme have been determined and are in good agreement with published results. Conditions for storing haemolysates at −20°C are described. The method is suitable for semi-automation.

Publisher

SAGE Publications

Subject

Clinical Biochemistry,General Medicine

Link

http://journals.sagepub.com/doi/pdf/10.1177/000456328402100505

Reference15 articles.

1. Gout with purine overproduction due to increased phosphoribosylpyrophosphate synthetase activity

2. Regulation of the synthesis of 5-phosphoribosyl-i-pyrophosphate in intact red blood cells and in cell-free preparations

3. Evaluation of the role of 5-phosphoribosyl-α-1-pyrophosphate synthetase in congenital hyperuricemia and gout: A simple isotopic assay and an activity stain for the enzyme

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